| Conditions | |
|---|---|
| Column | Kinetex C18 (4.6 × 250 mm, 5µ, Phenomenex) |
| Guard column | UHPLC C18 (AJ0-8768, Phenomenex) |
| Column Temperature | 25 °C |
| Mobile phases | A (0.1% phosphoric acid 30) : B (Acetonitrile 70) |
| Flow rate | 1.0 mL/min |
| Wavelength | 237 nm |
| Injection volume | 10 µL |