Table 5.
Validation of robustness depending on solvent manufacturer, flow rate, and column temperature
| Retention time (min) | Area (µAU) | Ascorbic acid concentration (μg/mL) | Ascorbic acid (%) |
| Solvent manufacturer | A | 2.62 (0.00) | 106010 (450) | 0.56 (0.02) | 74.14 (3.02) |
| B | 2.61(0.00) | 105796 (287) | 0.55 (0.01) | 73.86 (1.46) |
|
| Average | 2.62 | 105903 | 0.55 | 74.00 |
| SDa | 0.01 | 357 | 0.02 | 2.13 |
| RSDb | 0.29 | 0.34 | 2.79 | 2.87 |
| Flow rate (mL/min) | 0.6 | 4.32 (0.00) | 190584 (5261) | 0.51 (0.01) | 53.57 (5.57) |
| 1.0 | 2.62 (0.00) | 105983 (322) | 0.56 (0.01) | 75.44 (0.28) |
|
| Average | 3.47 | 148284 | 0.53 | 64.51 |
| SD | 0.93 | 46458 | 0.03 | 12.49 |
| RSD | 26.82 | 31.33 | 4.90 | 19.36 |
| Column temperature (°C) | 30 | 2.62 (0.00) | 106270 (685) | 0.57 (0.03) | 76.27 (0.89) |
| 35 | 2.60 (0.00) | 106028 (895) | 0.55 (0.04) | 74.50 (3.82) |
|
| Average | 2.61 | 106149 | 0.56 | 75.38 |
| SD | 0.01 | 725 | 0.02 | 2.66 |
| RSD | 0.46 | 0.68 | 3.47 | 3.53 |
Standard deviation
Relative standard deviation (%)
The parenthesis contains the standard deviation.