Table 2.
The enzyme activity of isotig01043 protein expressed in E. coli with pET-24a(+). The second elution of Ni-column purification was used for the assay of enzymatic activities

Enzyme	Substrate	Enzyme activity (U/ml)
Cellobiohydrolase	10 mM p-nitrophenyl-β-D-cellobioside	N.D.
β-glucosidase	10 mM p-nitrophenyl-β-D-cellobioside	N.D.
Exo-1,3-1,4-glucanase	1% Barley beta-glucan	N.D.
Endo-β-1,4-xylanase	10 mg/ml Beechwood xylan	0.235
Xylan-1,4-β-xylosidase	10 mM 4-Nitrophenyl β-D-xylopyranoside	N.D.
Chitosanase	0.2% Chitosan	N.D.
Endo-glucanase	2% Carboxymethyl cellulose	N.D.

N.D.: not detectable